Live-dead stain environmentally-grown  A.thaliana.

Live-dead stain environmentally-grown A.thaliana.

ORGANISATION:
The University of Canterbury
LOCATION:
School of Biological Sciences, Christchurch
CONTACT PI: Mitja Remus-Emsermann [email]
WEBSITES:
University of Canterbury - Mitja Remus-Emsermann
Laboratory - Remus-Emsermann Lab

About

The Remus-Emsermann Laboratory is principally interested how bacterial species grow on plant leaves, which growth pattern they exhibit and how they interact with other species that share the leaf with them. To do so, we mostly rely on fluorescence microscopy techniques such as confocal laser scanning microscopy and widefield epifluorescence microscopy. This in combination with bacteria that are either tagged with fluorescent protein genes, stained using fluorescence in situ hybridisation (FISH), or stained using fluorescent live/dead stains that allow to determine if individual bacterial cells are either alive or dead. To analyse our data we employ image cytometry and spatial statistic tools which allow us to assess pair aggregation patterns of leaf colonising bacteria.

LABORATORY RESEARCH FOCUSES

  • microbial ecology of plant leaf surfaces (phyllosphere microbiology) using single-cell resolution tools and plant microbe interactions

STUDENTS AND STAFF
Staff and Post-docs: Dr. Daniela Remus
Postgraduate students: Simisola Oso, Rudolf Schlechter, Michal Bernach, Erica Boyd

SELECTED PUBLICATIONS

Remus-Emsermann MNP, Schlechter RO. 2018. Phyllosphere microbiology: at the interface between microbial individuals and the plant host. New Phytol, doi: https://doi.org/10.1111/nph.15054

Freimoser FM, Pelludat C, Remus-Emsermann MN. 2016. Tritagonist as a new term for uncharacterised microorganisms in environmental systems. ISME J, 10, 1-3, doi: https://doi.org/10.1038/ismej.2015.92.

Remus-Emsermann MN, Lucker S, Muller DB, Potthoff E, Daims H, Vorholt JA. 2014. Spatial distribution analyses of natural phyllosphere-colonizing bacteria on Arabidopsis thaliana revealed by fluorescence in situ hybridization. Environ Microbiol, 16, 2329-2340, doi: https://doi.org/10.1111/1462-2920.12482.

Remus-Emsermann MN, Tecon R, Kowalchuk GA, Leveau JH. 2012. Variation in local carrying capacity and the individual fate of bacterial colonizers in the phyllosphere. ISME J, 6, 756-765, https://doi.org/10.1038/ismej.2011.209.

Remus-Emsermann MN, Leveau JH. 2010. Linking environmental heterogeneity and reproductive success at single-cell resolution. ISME J, 4, 215-222, doi: https://doi.org/10.1038/ismej.2009.110.